Rapid Assay for detection of Human Cardiac Troponin I (cTnl) in Serum/Plasma & Whole Blood by
ImmunoChromatography
Fig. Troponin I kit showing Positive result |
Principle:
This test utilizes the principle of
immunochromatography, with a unique two-site sandwich immunoassay on a nitrocellulose membrane. The conjugate
pad contains two components - monoclonal
anti-cTnl conjugated to colloidal gold
and rabbit IgG conjugated to colloidal gold. As the test sample flows through
the membrane assembly of the device,
the highly specific anti-cTnl antibody - colloidal gold conjugate complexes with cTnl in the sample and travels on the membrane due to capillary action
along with rabbit IgG-colloidal gold conjugate.
This sample moves further on the membrane to the test region (T) where it is
immobilized by another specific
anti-cTnl antibody coated on the membrane leading to the formation of a
pink-purple band. A detectable colored
band is formed if cTnl level is equal to or greater than 0.1 ng/ml. The absence of this colored
band in the test region indicates cTnl concentration < 0.1 ng/ml.