Tuesday, May 24, 2016

Cardiac Troponin I (cTnI) assay by immunochromatography method: Principle, Procedure, Interpretation and results

Rapid Assay for detection of Human Cardiac Troponin I (cTnl) in Serum/Plasma & Whole Blood by ImmunoChromatography

Fig. Troponin I kit showing Positive result
This test utilizes the principle of immunochromatography, with a unique two-site sandwich immunoassay on a nitrocellulose membrane. The conjugate pad contains two components - monoclonal anti-cTnl conjugated to colloidal gold and rabbit IgG conjugated to colloidal gold. As the test sample flows through the membrane assembly of the device, the highly specific anti-cTnl antibody - colloidal gold conjugate complexes with cTnl in the sample and travels on the membrane due to capillary action along with rabbit IgG-colloidal gold conjugate. This sample moves further on the membrane to the test region (T) where it is immobilized by another specific anti-cTnl antibody coated on the membrane leading to the formation of a pink-purple band. A detectable colored band is formed if cTnl level is equal to or greater  than 0.1 ng/ml. The absence of this colored band in the test region indicates cTnl concentration < 0.1 ng/ml.

Related Posts Plugin for WordPress, Blogger...